Thermo Fisher Scientific has introduced two additional systems designed to advance intact protein analysis and ‘quanfirmation.’ The new technology includes: Extended Mass Range (EMR) option for the Thermo Scientific Exactive Plus LC-MS and Thermo Scientific Q Exactive Plus LC-MS. Apart from these, Orbitrap Fusion Tribrid LC-MS system and TSQ Quantiva triple stage quadrupole LC-MS system were also launched. All the products were displayed at 12th HUPO World Congress at the Pacifico Yokohama in booth 51.
Thermo Scientific Exactive Plus LC-MS: Proteomics scientists have a tool for challenging high resolution accurate mass (HRAM) intact protein analysis such as investigating the structure, topology and architecture of targets. These include: impurities in monoclonal antibodies, antibody-drug conjugates, PEGylated proteins, oligomerised protein-based drugs, glycoforms and protein assemblies. The EMR option for the Thermo Scientific Exactive Plus LC-MS includes:
- Extended m/z range of 350-20,000
- Improved transmission of higher-mass ions for stronger signals
- Modified HCD pressure and controls for easier optimisation of experimental conditions
- Access to long transients for improved signal-to-noise ratio
| Q Exactive Plus LC-MS |
Thermo Scientific Q Exactive Plus LC-MS: The Thermo Scientific Q Exactive Plus LC-MS will further increase performance for applications such as bottom-up proteomics, lipidomics and high-confidence DMPK qual/quan screening studies.
Enhancements include advanced Quadrupole Technology (AQT) which has been developed to improve precursor selection and transmission for more-accurate quantitation of low-abundance analytes in complex matrices. Sophisticated data-independent acquisition (DIA) and parallel reaction monitoring (PRM) designed to deliver reproducible, high-throughput quantitation with very high confidence in qualitative results.
Advanced active beam guide (AABG) has been designed to reduce noise and extend maintenance intervals. Optional protein mode designed to permit greater control over collision pressure and related parameters to improve analysis of intact proteins and protein complexes. Enhanced resolution option intended to increase ID confidence in top-down proteomics and lipidomics studies.
| Orbitrap Fusion |
Thermo Scientific Orbitrap Fusion Tribrid LC-MS system is a novel tribrid configuration which enables users to positively identify larger numbers of low-abundance proteins faster than previously possible with existing commercial instruments. Its unique architecture enables simultaneous precursor isolation, fragmentation, and data acquisition in both the Orbitrap and linear ion trap mass analysers. More high-quality data can be collected compared to previous instruments, expanding the range of possible experiments.
| Exactive Plus EMR LC-MS |
Attributes include: A quadrupole for precursor selection at isolation widths down to 0.4 amu for excellent sensitivity and selectivity; An ultra-high-field Orbitrap offering resolution in excess of 450,000 and scan rates up to 15 Hz for unsurpassed selectivity and speed of analysis; An ion routing multipole followed by dual-pressure linear ion trap providing MSn HCD, CID and ETD fragmentations and fast, sensitive mass analysis with scan rates of up to 20 Hz. Synchronous precursor selection enhances the instrument’s signal-to-noise performance.
| TSQ-Quantiva-Nanospray-Easy-nLC-Front |
The new Thermo Scientific TSQ Quantiva triple stage quadrupole LC-MS system has been designed to transform quantitation experiments with extreme sensitivity, productivity, precision and usability. The TSQ Quantiva system breaks the attogram sensitivity barrier, thanks to its new Active Ion Management (AIM) design. It is also extremely productive, able to perform 500 SRM experiments per second and positive/negative polarity switching in 20ms with no signal loss.
Thermo Scientific TSQ Endura triple stage quadrupole LC-MS shares much of the advanced technology of the TSQ Quantiva MS, while also designed to deliver higher uptime than any competitive triple quadrupole instrument in workhorse applications requiring trace level quantitation. Thermo Fisher Scientific Pierce Tandem Mass Tag 10-Plex Isobaric Mass Tag Labeling Kits and Reagents for multiplexed protein identification and quantitative analysis by triple stage quadrupole mass spectrometry. Thermo Scientific myECL Imager, a powerful and easy-to-use blot and gel documentation instrument for sensitive, multimode image capture and analysis via an intuitive touch screen interface and advanced integrated software.
Contact Details:
Stuart Matlow
Public Relations Manager
Chromatography and Mass Spectrometry
Thermo Fisher Scientific
355 River Oaks Parkway
San Jose, CA 95134
Office: (408) 965-6408
Mobile: (415) 407-5474
Email: stu.matlow@thermofisher.com
Website: www.thermofisher.com